Effects of epimedium pubescens icariin on proliferation and differentiation of human osteoblasts
Epimedium belong to Epimedium macranthum Morr.et Decne, Epimedium brevicornum Maxim, Epimedium
sagittatum Maxim, berberidaceae plant . Also named epimedium gradiflorum morr . Mainly include:
flavonoids, glycosides and polysaccharide. Many researches have show the total flavonoids of epimedium have
the good control effect to the ovary removal of rats caused by osteoporosis, and promote osteoblast proliferation
and differentiation of rats in vitro.
In order to get the exact function of epimedium how to control osteoporosis, this study mainly used the icariin
( mainly include the purity of icariin 10%, icariin 20%, icariin 98% and etc) as the object of study, proliferation
and differentiation as the index to observe the influence of the osteoblast. Human osteoblasts were obtained by
inducting human marrow mesenchymal stem cells (hMSCs) directionally. MTT was used to observe the
proliferation and activity of ALP was assayed to observe the differentiation of the third passage human osteoblasts
cultured in vitro. The expression of BMP-2 mRNA was checked by RT-PCR. Result: Epimedium pubescens icariine
at the dose of 20 μg·mL-1 increased greatly the proliferation and differentiation of human osteoblasts and promoted
the expression of BMP-2 mRNA.
Then from this study showthat epimedium pubescens icariin enhances isgnificantly the proliferation and
differentiation of human osteoblasts, which may be mediated by increasing the expression of BMP-2 mRNA.